For scripts

1. Place all scripts in the main folder.
2. Ensure all scripts have parameters.
3. Ensure all scripts can process multiple data sets.

For file and folder names

• If the table has all contigs, the name will start with All. If the table only has part of the information, like contigs with defense records, the name will start with all.
• The second word in the name should reflect the table's subject. If the main ID in the file is contig, it should be Contig.
• Use “_” to connect all file names.
• Rename all file contents from - to _.
• During the merging process, remove duplicate titles.
• Remove outliers from the samples. Similarly, but mark why it is an outlier.
• Sequence
• Table: file
• Statistic_file: the file can be used to draw pictures.
• Use csv format to store data.

For file and folder save location

• Place the original tables on Google Drive.
• Place the results in the results folder.

For Bioinformatics data preparation

For FASTA file